Journal: Cell reports

Article Title: Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population

doi: 10.1016/j.celrep.2019.11.042

Figure Lengend Snippet: (A) Splenic DCs were analyzed 6 h after intravenous (i.v.) inoculation of CpG-A (filled bars) or PBS control (empty bars). gMFI of activation markers is shown as the mean ± SD (n = 2–4 in 2–4 exp). See for histograms of activation markers. (B) Human DCs were sorted from PBMCs and analyzed at time 0 (empty bars) or after 24 h of culture with 5 μg/mL of CpG-A (filled bars). gMFI of activation markers is shown as the mean ± SD (n = 2–4 in 2–4 exp). (C) IFNα and IL-12p70 measured by ELISA in supernatants from sorted mouse DCs stimulated with CpG-A for 16–18 h (n = 4 in 4 exp). Frequency of IL-12p40-positive cells was measured by intracellular cytokine staining after 4 h of stimulation with CpG-A in the presence of brefeldin A (BFA). (D) IFNα measured by ELISA and IL-12p70 measured by cytometric bead array in the supernatants from sorted human DCs stimulated as in (B) or with an adjuvant cocktail (lipopolysaccharide-poly(I:C)-R848 [LPR]). (E) Frequency of CellTrace violet (CTV) low mouse CD4 + T cells in mixed leukocyte reactions. DCs were sorted from B6 mice and cocultured with CTV-labeled CD4 + T cells from BALB/c mice for 5 days (n = 3 in 3 exp). (F) Frequency of carboxyfluorescein succinimidyl ester (CFSE) low human CD4 + T cells in mixed leukocyte reactions. Human DC populations were sorted and cocultured with allogeneic CFSE-labeled T cells for 5–6 days (n = 3–4 in 3 exp). Bar graphs indicate mean ± SD; nd, not detected. Statistics determined by one-way ANOVA with Tukey’s multiple comparison test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: Human IFN Alpha Multi-Subtype ELISA Kit (TCM) , PBL Assay Science , Cat# 41105-1.

Techniques: Control, Activation Assay, Enzyme-linked Immunosorbent Assay, Staining, Adjuvant, Labeling, Comparison

Journal: Cell reports

Article Title: Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population

doi: 10.1016/j.celrep.2019.11.042

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Human IFN Alpha Multi-Subtype ELISA Kit (TCM) , PBL Assay Science , Cat# 41105-1.

Techniques: Purification, Virus, Recombinant, Lysis, Electron Microscopy, Transfection, Labeling, Staining, Cell Isolation, Enzyme-linked Immunosorbent Assay, Control, Antibody Labeling, Reverse Transcription, Single Cell, RNA sequencing, Software